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The ATP level of the sample was detected by an Enhanced ATP Assay Kit (S0027, Beyotime)

Finally, important molecules related to the cell cycle were assessed via western blot assays

In addition to regulation by hormone, recent work has also highlighted the importance of the balance between positive (Sp1) and negative (Pur) transcription factors binding to the 5UTR of the human gene in determining the expression of receptor mRNA in different prostate cancer cell models (52 and references therein)

& Yi, S

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however, percent body fat mass trended higher [12]