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Specific primers ( Table S2 ) were used to detect the human mRNAs of CCL1 (C-C Motif Chemokine Ligand 1) (NM_002981.2), CCL2 (C-C Motif Chemokine Ligand 2) (NM_002982.4), CCL3 (C-C Motif Chemokine Ligand 3) (NM_002983.3), CXCL9 (C-X-C motif chemokine ligand 9) (NM_002416.3), IL1B (Interleukin 1 beta) (NM_000576.3), IL6 (Interleukin 6) (NM_000600.5), IL17A (Interleukin 17A) (NM_002190.3), TNF (Tumor Necrosis Factor) (NM_000594.4), HAVCR2 (Hepatitis A Virus Cellular Receptor 2) (NM_032782.5), CD4 (CD4) (NM_000616.5) and CD8A (CD8a) (NM_001768.7)

The entire supernatant was transferred to a new tube and dried in a SpeedVac

Cachexia pathophysiology: Driven by pro-inflammatory cytokines TNF-, IL-1, IL-6, IFN- released by the underlying disease (tumor, failing heart, inflamed lung)

Neuronal compartmentalization results in impoverished axonal mitochondria