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Van de Wiele, T

The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm

Accumulation of cytosolic succinate results the inhibition of the 2-oxoglutarate and Fe 2+ -dependent dioxygenases [prolyl hydroxylase domain (PDH) enzymes] responsible for the hydroxylation of the -subunit (HIF-1) of HIF-1
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