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Importantly, the development of 2pFLIM on organotypic slices has allowed exploring the spatiotemporal dynamics of biochemical signaling in dendritic spines, and a proof-of-principle in vivo has been published (Mower et al., 2011)

Evaluation of porosity Six specimens were used for each material to assess porosity: three dry-stored specimens and three specimens after being immersed in distilled water for 180 days
