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DNA template for each clone was amplified from the bacterial culture in a glycerol stock 384-well plate using a TempliPhi DNA amplification kit (GE Healthcare)

The expression of target genes was analyzed via quantitative real-time (RT) PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)

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10.1177/0884533614530170 [DOI] [PMC free article] [PubMed] [Google Scholar] Skovbakke S

386,387 After myocardial I/R injury, the activation of Wnt/-catenin signaling can promote YAP1 transcription, thereby inhibiting Hippo-YAP signaling and subsequently suppressing cell hypertrophy induced by myocardial I/R injury 142 (Fig