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By age 40 yr, there is a ubiquitous and significant occurrence of neuropathological hallmarks of AD, as indicated by widespread deposition of senile plaques and neurofibrillary tangles, but dementia is variable
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After electrophoresis, the gel was stained with Coomassie Brilliant Blue or transblotted onto a PVDF membrane and incubated with 0.25% polyvinylpyrrolidone (Sigma, St
