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Description
Five microliter of the prepared sample was injected onto a Kinetex 2.6 m 100 100 2.1 mm (Phenomenex, Torrance, CA, USA) using SIL-30 AC auto sampler (Shimazdu, Columbia, MD, USA) connected with a high flow LC-30AD solvent delivery unit (Shimazdu) and CBM-20 A communication bus module (Shimazdu) online with QTRAP 5500 (Sciex) operating in positive and negative ion mode

The first escalation doubles the dose, which sounds aggressive but represents the smallest absolute increase in the protocol

Atherosclerosis has been a major contributor to the global burden of cardiovascular disease, which remains one of the leading causes of mortality worldwide [3]
Fever-driven infections cause sweat loss you do not notice Any illness with elevated body temperature loses fluid through sweat and faster breathing

10.1371/journal.pone.0065719 77 LundinN
Morel M, Bravo R, Espargar A, Sisquela X, Avils FX, Fernndez-Busquets X et al