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The suitable extraction conditions for globulin were determined as follows: extraction time of 110 min, solid-liquid ratio of 1:47 g/mL, extraction temperature of 37 C, and NaCl concentration of 1.24 mol/L

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J Colloid Sci 4:425437 Smoluchowski MV, Einer V (1918) Mathematischen Theorie Der Koagulations kinetik Kolloider Losungen