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Aortic tissue LOXL2 activity assay Aortas from young ( 18 months old) WT and LOXL2+/ littermates were extracted and cleaned free of connective tissue in Krebs-HEPES buffer (NaCl 135 mM, KCl 5 mM, MgSO 4 1 mM, K 2 HPO 4 0.4 mM, Glucose 5.5 mM, HEPES 20 mM, adjust the pH to 7.4, supplement with 1 mM CaCl 2 ) containing 0.25 g/mL fungizone (Gibco)

Tucker KL, Mahnken B, Wilson PW, Jacques P, Selhub J

Monitor for adverse reactions during and after administration

Eur J Immunol 38:20852089

If someone has trouble swallowing pills, then it is generally recommended that he/she try chewable forms of Vitamin B12 such as Miduty Methyl B12 & Folate

If you've been consistent with daily dosing, proper reconstitution, and refrigerated storage, and still see zero change at 16 weeks, the issue is likely absorption or individual receptor sensitivity rather than insufficient dose
