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Nrf2: a promising therapeutic target for glucocorticoid-resistant chronic rhinosinusitis

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The membranes were blocked with 5% nonfat milk for 1 h at room temperature and then incubated with specific antibodies against GPX1 (1:2000, Proteintech, USA), GADPH (1:20000, Cell Signaling Technologies, USA), phospho-Src family (Tyr416), phospho-PI3 kinase p85 (Tyr458)/p55 (Tyr199), phospho-Akt (Ser473), and phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:2000, all from Cell Signaling Technologies, USA) overnight at 4 C

Rights and permissions About this article Cite this article Hollander, D., Kaunitz, J.D

After reconstitution, store at 2-8C and use within 30 days