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It has been demonstrated that small-molecule oral 2-oxoglutarate analogs inactivate HIF-PH in the presence of oxygen, serving as HIF stabilizers

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doi: 10.1016/j.mrrev.2009.12.005 117 KasaiH

To enhance the signal, sections were incubated in ABC solution (VectorLabs, catalog #PK-6100) for 1 h followed by a DAB solution (DAB, Sigma-Aldrich, catalog #91-95-2, 0.05%) of cobalt chloride (0.05%) and nickel ammonium sulfate (0.05%) to produce a black precipitate in c- fos + cells

Drop from twice weekly to once weekly

Start: Begin at 0.25 mg total blend once weekly (Standard) or 0.5 mg (Advanced) to gauge tolerability