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Glutathione undergoes a reaction with the toxic substances, facilitated by enzymes such as glutathione S-transferases, to form a conjugate known as a glutathione adduct, which is then further metabolized in the liver to form mercapturic acid, a water-soluble compound that can be easily excreted through the urine

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However, GCLC alone does have enzymatic activity as Gclm knockout mice are viable but have markedly reduced tissue GSH levels (reduced by about 85 to 90%) [44]

Trends Anal Chem (TrAC) 30(4):652664 Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase

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Moreover, oxidative stress is a common feature in many chronic diseases with long-term iron deficiency