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NHDF were cultivated in Dulbeccos modified Eagles low glucose medium (DMEM) supplemented with 10% FBS, glutamine (0.3 mg/mL), glucose (4 mg/mL), penicillin (100 units/mL), and streptomycin (0.1 mg/mL) in 75 cm 2 culture flasks under 5% CO 2 and at 37 C until the fifth passage