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The extracts were purified on SepPak 500 mg silica cartridges (Waters), concentrated in SpeedVac to a final volume of 200 L, and analyzed by GC-MS as described.26 Carbonyl compounds.The content of the 2 DNPH-filled impingers (see smoke collection procedure above) was combined and analyzed for 8 carbonyl compounds by HPLC-UV.27,28 Briefly, an aliquot of the DNPH solution was mixed with 1% Trizma base solution to quench the DNPH reaction

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Some of the possible effects are: Irritation and inflammation of the lungs can lead to wheezing, coughing, shortness of breath, and chest pain

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