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Estimated mRNA Copy Number Per Cell by smFISH Using the copy numbers of hu and cckA mRNAs determined by smFISH, we performed a linear regression between the mRNA copy number and RPKM values of the RNA-Seq data from cells grown in M2G media 17 (mRNA copy number = 0.0011 * RPKM R 2 =0.9952)

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