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66,67 Multiple studies carried out in a variety of systems using different techniques ranging from the UvrABC nuclease incision method in combination with ligation-mediated PCR to mass spectrometry of specific isotopically labeled TP53 sequences demonstrate that PAH diol epoxide metabolites react preferentially at the TP53 hotspots observed in tobacco smoke associated lung cancers

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While longitudinal studies take time, global surveillance of ST products, their chemical composition and risk profile can help improve the precision of future estimates