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Quantitative real-time PCRs were done with FastStart Universal SYBR Green Master Mix (Roche, Basel, Switzerland) and ABI 7300 thermal cycler (Applied Biosystems, Foster City, CA, USA), or with PowerUp SYBR Green Master Mix (Applied Biosystems) and QuantStudio 5 real-time qPCR thermal cycler (ThermoFisher Scientific)

Next, senescence-associated macrophages were recruited to the arterial wall, where the plaque initially formed

In ASD children, this reaction is impaired by a reduction of vitamin B12 levels probably attributed to a reduced oral intake, gut dysbiosis or autoimmunity