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In this step, RPMI culture medium (Bio-IDEA), which is suitable for PBMCs cell culture, along with FBS and trypan blue dye, was utilized to identification of living and dead cells

Finally, the historical trajectories of divergence between life expectancy with and without smoking-attributable mortality that we found are broadly similar to those previously found for specific countries [40]

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Exposure of human skin to benzo[a]pyrene: role of CYP1A1 and aryl hydrocarbon receptor in oxidative stress generation
