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The supernatant was filtered through a 220 nm membrane, and the protein was purified using the Ni-affinity chromatography (GE, USA) with the following buffers and the purified protein was analyzed using the SDS-PAGE

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The new device uses real tobacco refills, but instead of burning it to produce hazardous smoke and tar, it heats it to produce tobacco-flavored vapor

Scientists answer Tucker Carlson's claim"