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Basolateral media was changed every other day and the apical surface of ALI cultures was unwashed to preserve the accumulated mucus during the experiments

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Symptoms of chronic bronchitis may include: cough (called smokers cough) excessive mucus production coughing up mucus wheezing chest discomfort or tightness shortness of breath crackling sounds while breathing More severe effects that might appear as the condition worsens include: bluish lips, fingernails, and skin because of reduced oxygen levels swollen feet heart failure Treatment for chronic bronchitis aims to relieve symptoms, prevent complications, and slow the progression of the disease

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