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Besides the induction of inflammatory macrophages, nicotine treatments promote the M2 polarization, reduces the secretion of several proinflammatory cytokines in macrophages, and suppresses the alveolar macrophages to present the inhaled antigen to lymphocytes [130, 131]

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Chromatin Immunoprecipitation (ChIP) assay ChiP analysis was performed according to manufacturers instructions using the EZ-ChiP kit (Upstate Biotechnology,Inc., Lake Placid, NY, USA) in 16HBE cells stimulated with CSE (0% and 20%) in the presence or absence of GSK343 (1 M) as previously described 28

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doi:10.1016/j.drugalcdep.2019.04.011

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