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These results demonstrate that R2 retrotransposons mediate targeted insertion of full-length gene cassettes into rDNA safe-harbor loci in rice calli, supporting the development of a non-LTR R2 system for precise large-fragment insertion and enabling trait stacking and multigene engineering in cereals

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The pH meter was standardized after every procedure using a standard protocol, that is, using pH calibration solutions ranging from pH 4 to pH 7 [Figure 2]

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