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doi: 10.3390/dairy4040044 4

The requirements of B2 B cells were found to differ from those of B1 or MZ B cells [120], and the same loss-of-function mutation had a more markedly nonredundant effect on T cells [121]

2.15 Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions

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