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Description
Cloning and quantitative real-time PCR (qRT-PCR) analysis of NtHQT genes The coding sequences of NtHQT1-4 were cloned by PCR amplification from cDNA isolated from tobacco leaves and roots with gene-specific primers (Supplementary Table S1)
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The cultivation of Corojo and its hybrids for the production of the cigar wrapper takes place under cotton cloths, called tapados, which men on stilts erect in order to protect the tobacco against too much sun, wind and parasites