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For this, 1 L of sample was injected through a 2.0- L sample loop using the partial loop injection mode onto a Waters ACQUITY UPLC HSS T3 column, 1.8 m, 2.1 100 mm, and eluted at 0.32 mLmin 1 with 0.10% formic acid in water (A) and 0.10% formic acid in methanol:acetonitrile (2:3) (B) as solvents

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