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November 3, 2025

It has been shown that marijuana smokers require a higher dose of certain commonly used anesthetics

After incubation, the coverslip containing the cells were washed with PBS, fixed in Paraformaldehyde 4% for 15 min RT, then washed in PBS and permeabilized for 5 min with Buffer (0.05% Saponin in PBS- plus 3% BSA) and then incubated with blocking Solution (BSA 0,5% in PBS) 1 h RT

Or, as Dockrell put it: Careful, research-informed policy design is essential to tackle death, disease, and disparities without creating new problems for public health. Vaping & illicit drugs: Promises and pitfalls Dr Tom Freeman Director, Addiction and Mental Health Group, University of Bath James Dunworth Freeman started by pointing out that the main harm from cannabis is from smoking and if people vaped it instead of smoking, it would be significantly safer