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After 20 min of additional stirring, the reaction solution was transferred to a separatory funnel

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Specific primers for each gene (Supplementary Table 3) were designed using Primer Express software (Applied Biosystems)

Constantly Evolving Landscape It will be important to understand how these findings translate from the controlled setting of a clinical trial to the real world where people use e-cigarettes in various ways, including without behavioral support for cessation, and for reasons other than quitting smoking, Dr