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After incubation, the coverslip containing the cells were washed with PBS, fixed in Paraformaldehyde 4% for 15 min RT, then washed in PBS and permeabilized for 5 min with Buffer (0.05% Saponin in PBS- plus 3% BSA) and then incubated with blocking Solution (BSA 0,5% in PBS) 1 h RT

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When comparing these two, there are a number of things you must consider: Consumption method : Dipping tobacco is generally maximized by placing one between the gum and cheek, allowing its nicotine to be released and absorbed by your mouths lining