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hirsute (51.56%) ( Bacillus amyloliquefaciens SL-7 (28.55%) ( The role of microorganisms in the fermentation process of tobacco leaves is closely related to the enzymatic reaction, as microorganisms secrete various enzymes (e.g., alpha-amylase (Ullah et al., 2021), protease (Yogesh and Halami, 2015), cellulase ( Bacillus amyloliquefaciens LB with high alpha-amylase activity and Bacillus kochii SC with high neutral protease activity has been used to improve sensory quality of flue-cured tobacco (Wu et al., 2021)