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The membranes were washed with Tween-Tris-buffered saline (TTBS) (10 mmol/l Tris-HCl, pH 7.4, 150 mmol/l NaCl containing 0.1% Tween-20), then blocked for 1 h with Tween-TBS (containing 5% non-fat dry milk), and incubated overnight at 4 C with primary antibodies prepared in TTBS containing 5% bovine serum albumin (BSA)

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