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The American Diabetes Association (ADA) recommends considering periodic measurement of vitamin B12 levels in patients with type 2 diabetes taking metformin, particularly those with anemia or peripheral neuropathy, though no specific monitoring interval is specified
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2.15 Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions
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