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Generation of YPEL5 -mutant cells HEK293T cells were transfected with either pN106/YPEL5-FLAG-WT, pN106/YPEL5-FLAG-T36A, pN106/YPEL5-FLAG-R41A or pN106/YPEL5-FLAG-L62A, along with pMD2.G (Addgene, 12259) and psPAX2 (Addgene, 12260), using TransIT-LT1 transfection reagents (Mirus Bio) to produce lentivirus

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Baker DW, Williams MV, Parker RM, Gazmararian JA, Nurss J

3,4 A systematic analysis of 52 countries (INTERHEART) showed that current smokers had a 2.95-fold higher risk of acute myocardial infarction compared with never smokers

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