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After being blocked by 3% BSA in PBS for 60 min at room temperature, cells were incubated with Goat anti-Human Albumin Antibody (Bethyl Laboratories, Inc.) for 2 h at room temperature, washed three times with TBST, and then incubated with Cy3-conjugated AffiniPure Donkey Anti-Goat secondary antibody (Jackson) for 60 min at room temperature in dark