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Portincasa P, Bonfrate L, Vacca M, De Angelis M, Farella I, Lanza E, et al

Theres evidence that nicotine can harm brain development in young people, and nearly 90% of current smokers started smoking before age 18
Isolated RNA was normalized to 3.5 ng input/sample for automated library preparation with the Ion AmpliSeq Transcriptome Human Gene Expression Kit, using 12 cycles of amplification
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