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A 2014 audit report reviewing Animal Welfare Act oversight of laboratories found that animals are not always receiving basic humane care and treatment and, in some cases, pain and distress are not minimized during and after experimental procedures. The animals most commonly used in experimentspurpose-bred mice and rats (mice and rats bred specifically to be used in experiments)are not counted in annual USDA statistics and are not afforded the minimal protections provided under the Animal Welfare Act

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In addition, in vitro and in vivo studies found that cigarette smoke not only decrease the fecal abundance of Bifidobacterium but also reduce its production of short chain fatty acids (SCFAs) [124, 173], immune-regulatory molecules modulating immune and inflammatory response within many diseases, and reductions in the concentration of SCFAs especially butyrate in colorectal tissues were demonstrated to be associated with the possibility of early stage CRC development [174]

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