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Then, 10 mL of acetonitrile (chromatographical grade) and 50 L of internal standard working solution (phenethyl acetate: 9.06 mg mL 1 ) was pipetted into the centrifuge tube, which was shaken at 2000 rpm for 120 min in a vortex oscillator and cooled at 4 C for 10 min subsequently

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All manure leaves were flue-cured in sequential batches with three steps of yellowing, fixed color, and dry gluten period according to Rules for curing technique of flue-cured tobacco [19]

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