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[82, 83] randomised 657 adult smokers wanting to quit to 16 mg nicotine e-cigarettes (as needed), 21 mg nicotine patches (one per day), or placebo e-cigarettes (no nicotine, as needed) in a 4:4:1 ratio

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The genomic DNA was isolated from the spermatozoa using the QIAamp DNA Mini Kit, followed by mitochondrial DNA amplification via the REPLI-g Mitochondrial DNA Kit supplied by QIAGEN (Hilden, Germany), adhering to the kits instruction manual

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