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Figure 5 Correlation between burden and SDI To explore the role of the SDI in the burden of substance use, we generated scatter plots to illustrate the correlation between SDI and both the EAPC and ASDALYR over the past three decades (Figure 6)

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The coverslips were flipped over onto ProLong diamond antifade mountant with DAPI (Invitrogen, CA, USA)-loaded slide glasses and incubated overnight at 4 C