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tzi the Iceman, a well-preserved mummy from around 5,000 years ago, for example, was found with 61 tattooed symbols across his body

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The tubes were incubated at 37C for 30 min, after which 10 l of proteinase K (20 mg/ml) and 50 l of 10% sodium dodecyl sulfate (SDS) was added to the tube and vortexed briefly