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1 x Make Your Own Machine 4 x Box of Make Your Own Tubes (100 tubes) 1 x 200gm Brookfield Volume Tubing Tobacco Note: You require approximately 0.5gm of tobacco per tube

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Further transcriptome analysis revealed 330 differentially expressed genes in the tenfold dilution group (T10_4h) and 438 genes in the 30-fold dilution group (T30_4h)