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Modulation of T-cell proliferation by stimulation of the -adrenergic receptor

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Then, 2 l of cDNA was used for qPCR using the SsoAdvanced Universal SYBR Supermix (BIO-RAD, Hercules-California, USA) in a 10 l reaction volume, following the manufacturers specifications, in a C1000 touch CFX96 real-time PCR system (BIO-RAD, Hercules-California, USA)