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In general, out of 67 variables detected significant by GBM, 11 variables (16%) correspond to individuals cigarette smoking history and cigarette use habits in wave 1, 11 (16%) are variables related to tobacco products use (other than cigarettes and e-cigarettes) in waves 1 and 2, 10 (15%) are physical and mental health and quality of life variables in wave 1, 9 (13%) are demographics and socio-economic status (SES) variables in wave 1, 7 (10%) are variables related to family and friends tobacco products use habits and rules about tobacco products use at home in wave 1, 5 (8%) are variables related to knowledge and beliefs about smoking and tobacco products harmfulness in wave 1, 4 (6%) are variables related to the exposure to tobacco ads and promotions in wave 1, 3 (5%) are alcohol and other substances use variables in wave 1, 3 (5%) are variables related to noticing tobacco products health warnings in wave 1, 2 (3%) are e-cigarette use variables in waves 1 and 2, and 2 (3%) are variables related to social media exposure in wave 1

RNA isolation and qRT-PCR Total RNA was extracted from cell lines with TRIzol Reagent (Invitrogen, CA, USA) following the manufacturers instructions, and was reverse-transcribed into cDNA, using M-MLV-RT and oligo(dT) primer (Invitrogen, CA, USA)

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