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The OAS was isolated from 5 g of wild-type TMV by RT-PCR in a reaction containing 0.2 M primer pLen_seq_rev, 200 U M-MLV Reverse Transcriptase RNaseH Minus Point Mutant (Promega, Walldorf, Germany), 1x M-MLV reaction buffer, and 1 mM dNTPs

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The term "blunt" is believed to have originated in the early 1980s on the streets of New York City

Much work remains in courts, legislatures, schools, and communities. Numerous parents who have attributed their childrens untimely deaths to social media attended the trial in L.A