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Cigarettes were then smoked on a Borgwaldt LX1 linear single port smoking machine under ISO (35-mL puff volume, 2-s puff duration and 60 s puff interval) and Canadian Intense (55-mL puff volume, 2-s puff duration, 30-s puff interval, and 100% blocked ventilation holes) smoking regimens.21,22 For the analyses of alkaloids, TSNA, beta-carbolines, and PAH, the mainstream smoke was collected on Cambridge filter pads

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