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To further assess the purity, in the third passage (activated PSCs) using immunohistochemistry to assess the co-expression of -SMA (marker of activated stellate cells) and the presence of a cell (Hoechst33342) using ImageJ (NIH)

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MS contributed to study design, collected and analyzed data, and wrote the manuscript

24 The treated subjects had similar age and HbA1c of that reported in Italy, although had a shorter duration of diabetes but with a higher cardiovascular burden (around 30% had established cardiovascular disease)

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