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Both enzymes, which are part of the BER pathway, leave the DNA with an abasic site, which is marked by an apurinic/apyrimidinic endodeoxyribonuclease (APEX1) that creates a single-strand break in the DNA

The high-risk category comprises adolescents who report past-year use along with two or more risk indicators, underscoring the need for comprehensive clinical evaluation and targeted intervention (23)