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Journal of Medical Internet Research

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Author Manuscript Author Manuscript Author Manuscript Author Manuscript Tobacco Filler Analysis Alkaloids and TSNA.For each cigarette, tobacco filler was removed from the cigarette rod and 200 mg were weighed and extracted in 10 mL of 10 mM ammonium acetate buffer by sonicating for one hour

The curing and processing also avoid chemical additives that commercial tobaccos may include

Researcher Corinne Gavard contributed to this report.