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Chromatin Immunoprecipitation (ChIP) assay ChiP analysis was performed according to manufacturers instructions using the EZ-ChiP kit (Upstate Biotechnology,Inc., Lake Placid, NY, USA) in 16HBE cells stimulated with CSE (0% and 20%) in the presence or absence of GSK343 (1 M) as previously described 28

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These 72 values were used as substitutes for the prevalence figures in the countries belonging to the group