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It could mean that antismoking efforts are effective at preventing young adults from lighting up and possibly developing a lifelong addiction

"The mortality of doctors in relation to their smoking habits: a preliminary report

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Reactions were performed in triplicate using the following conditions: one cycle at 95 C for 3 min, followed by 40 cycles at 95 C for 5 s and 60 C for 25 s, and a melting curve plot was constructed to confirm that no reaction produced non-specific amplification

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