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HLA complexes were immunoprecipitated by incubating 40 L per 5 10 7 cells of GammaBind Plus Sepharose Beads (Cytiva, 17088601), pre-charged for 3 h, with 100 g per 5 10 7 cells of anti-human MHC Class I (HLA-A, HLA-B, HLA-C) antibody (clone W6/32, BioXCell, BE0079) and rotating overnight at 4 C

B12 therapy for wellness purposes is typically not covered by insurance, but if prescribed by your physician for a diagnosed B12 deficiency, coverage may be available

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doi: 10.1158/2159-8290.CD-13-0015 243 HanMXuWChengPJinHWangX

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